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PD-L1 IHC TPS Score*

Egible for treatment**

Cell line controls***

1. Cell line 1

NA

NA

2. Cell line 2

NA

NA

3. Cell line 3

NA

NA

4. Cell line 4

NA

NA

Tissue controls

8. Tonsil

NA

NA

13. Tonsil

NA

NA

12. Placenta

NA

NA

NSCLC

5. NSCLC

No <1%

No

9. NSCLC

No <1%

No

14. NSCLC

No <1%

No

10. NSCLC

Low 1-49%

Yes

15. NSCLC

Low 1-49%

Yes

6. NSCLC

High ≥ 50%

Yes

7. NSCLC

High ≥ 50%

Yes

11. NSCLC

High ≥ 50%

Yes

16. NSCLC

High ≥ 50%

Yes

Material

The slides to be stained for PD-L1 comprised the following 16 materials

PD-L1:

1-4: Cell lines, 5-7, 9-11 & 14-16: Non small lung cell carcinomas, 8 &

13: Tonsil,

12: Placenta.

PD-L1 standardization provides

precision in assay results