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Diehl F, et. al Analysis of mutations in DNA isolated from plasma
and stool of colorectal cancer patients. Gastroenterology. 2008
Aug;135(2):489-98.
1%
100%
10%
0.1%
0.01%
Digital PCR (ex. BEAMing)
Real-Time PCR
Pyrosequencing
Sanger Sequencing
Detection Capability
(mutant DNA/ total DNA)
NGS
Mutant Allele Fraction (MAF) could be determined with Beaming (quantitative) technology.
However, its prognosis impact in CRC has not been explored.
LOW MAF
HIGH MAF
Background